From: HubMed - mesothelioma <rssfwd@rssfwd.com>
To: shell8377@yahoo.com
Sent: Thursday, February 14, 2008 10:44:13 PM
Subject: Phase-contrast microscopy studies of early Cisplatin-induced morphological changes of malignant mesothelioma cells and the correspondence to induced apoptosis.
[1]Exp Lung Res. 2008 Feb; 34(2): 49-67
Janson V, Behnam-Motlagh P, Henriksson R, Hörstedt P, Engström KG, Grankvist K
Cisplatin treatment efficacy of malignant pleural mesothelioma (MPM) is aggravated by resistance and adverse effects. In P31 MPM cells, cisplatin induces morphological changes and apoptosis. To determine if very early (10 minutes) morphological responses corresponded to apoptosis-induction, cisplatin effects on P31 morphology were examined with phase-contrast microscopy (PCM), scanning electron microscopy (SEM), and flow cytometry (fluorescence-activated cell sorting [FACS]), and compared to apoptosis-induction over time. Increased membrane protrusions were identified with PCM and SEM, but these were not consistent with the induction of apoptosis. The authors concluded that very early morphological changes can be determined with PCM in MPM, but they did not convincingly correspond to apoptosis induction.
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Source: http://www.hubmed.org/display.cgi?uids=18266129
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. Mesothelioma is the word used to describe a cancerous tumor that involves the mesothelial cells of an organ, often the lungs, heart, or abdominal organs.
In this disease, malignant cells develop in the mesothelium, a protective lining that covers transcendently of the spread's internal organs. Greatest malignant mesotheliomas set up complex karyotypes, with extensive aneuploidy and rearrangement of tons chromosomes.
Symptoms of mesothelioma may not appear until 20 to 50 years after exposure to asbestos. Diagnosing mesothelioma is often difficult, because the symptoms are similar to those of a number of other conditions. A history of exposure to asbestos may increase clinical suspicion for mesothelioma. A physical examination is performed, followed by chest X-ray and often lung function tests. The X-ray may reveal pleural thickening commonly seen after asbestos exposure and increases suspicion of mesothelioma.
If the cancer has length beyond the mesothelium to other parts of the size, symptoms may include pain, trouble swallowing, or swelling of the neck or engage.
Tissue is removed, placed under the microscope, and a pathologist makes a definitive diagnosis, and issues a pathology report. Mesothelioma is diagnosed by pathological examination from a biopsy.
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